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Phenotypical Characters in Hybrids Chicken of Crossbreeds between Pelung and Cemani

The germplasm variability of Indonesian local chicken is well known and one species known as “Ayam Pelung” originated from West Java is famous for its meat. The present study aimed to observe the phenotype characters i.e. fur color and body weight in crossbreed hybrids of Pelung and Cemani chicken as a potential meat-type breed. The parent stock, in this case, the Cemani

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Effect of Varying Level of Crude Protein and Energy on Insulin-like Growth Factor-I Expression Level in Indonesian Hybrid Chicken

The aims of this study were to investigate the effect of crude protein and energy level in feed on insulin-like growth factor-I (IGF-1) expression level in a hybrid chicken (female Layer chicken×male Pelung chicken). The weight highly correlated with the IGF-1 expression level (R2 = 0.8). This moderate growth response to nutrition makes the hybrid chicken

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Isolation of DNA from Chicken (Gallus gallus domesticus Linnaeus, 1758) Feather with Lysis Buffer-phenol Chloroform Isoamyl Alcohol Method (PCI) and Chelex Method

DNA extraction material can be obtained from the body part or body of a cell-containing organism. Feather is part of the cover body that contains cells in the base. The use of feathers as a sample of DNA isolation or DNA genome sources minimizes the stress on the chicken and simplifies the sampling process of many birds. The conclusions of this study were the concentration of isolate by chelex method higher than the buffer lysis-PCI method, the purity ratio of buffer

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Phenotypic Performance of Kambro Crossbreeds of Female Broiler Cobb 500 and Male Pelung Blirik Hitam

This research was conducted to measure the phenotypic performance of Kambro crossbreeds of Pelung Blirik Hitam and Broiler Cobb 500. Kambro has the phenotype combination of parental generation based on the phenotype parameter. Based on Body Weight (BT) measurement, Kambro population (n = 17) has an average BT of 1,244.14 ± 453.82 grams significant (p <0.01) to F1 Pelung (n = 7) with an average BT of 602.88 ± 79.93 grams in 8 weeks period with ad libitum diet of standard feed.

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Gama Ayam Research Team is one of research team based in Laboratory of Genetics and Breeding Faculty of Biology Universitas Gadjah Mada under the supervision of Prof. Dr. Budi Setiadi Daryono, M.Agr.Sc. Founded in 2008, Gama Ayam Research Team has a vision to breed, develop and preserve Indonesian native chicken germplasm in an effort to support Indonesia’s food fulfillment and create the independence chicken poultry farmers in particular.

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An experiment was conducted to detect SNP of the myostatin gene and its association with the body weight of hybrid chicken crossbreed from F1 Kamper and BC1 Broiler. Four F1 Kamper hens were crossbred with BC1 Broiler rooster. Day old chick (DOC) hatched were maintained for 49 days with body weight measurement every seven days. The blood samples from 49 days old chicken were taken for DNA isolation by Chelex 5% method and then amplification of the myostatin gene. PCR products were sequenced, and sequence alignment was performed using Clustal Omega to obtain SNP. The SNP obtained was analyzed by the Pearson correlation test with the body weight of forty nine-days-old chickens. The body weight of the hybrid chicken is higher than of Pelung chicken but lower than the Broiler. There are 7 SNPs in myostatin gene exons included 2 Adenine insertions, 1 Guanine deletion, and four substitutions (C2244G, G2283A, T4842G, G7378T) that yield nine haplotypes. Six haplotypes had different protein sequences with Myostatin protein, while three haplotypes were identical to Myostatin protein. The correlation analysis showed that there was a strong positive correlation (r = 0.736) between normal Myostatin protein and mutant to chicken body weight at 49-days-old. Adenine insertion to nucleotide 2099-2100 of myostatin gene had a very strong positive correlation (r = 0.800) to 49-days-old chicken body weight, although T4842G substitution had a strong negative relationship (r = -0.773) to 49-days-old chicken body weight. Adenine insertion to nucleotide 2099-2100 of myostatin gene could be a genetic marker of heavier body weight of the hybrid chicken.

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